Soluble CD14 Recombinant Protein

SKU: orb1230898

Description

Research Area

Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

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Key Properties

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Source	HEK293 cells
Biological Activity	Biological activity was determined by the dose dependent activation of NF - kappaB in a RAW264 cell line based reporter system, using a sCD14 concentration range of 20 ng/μL to 200 ng/μL. The NF - kappaB activation is enhanced when the assay is done in the presence of 0.25 ng/μL to 1.0 ng/μL bacterial LPS.
Target	CD14
Protein Sequence	TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P
Purity	Greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Storage & Handling

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Storage	The lyophilized Soluble CD14 recombinant protein is stable for at least 2 years from date of receipt at -20˚°C. Reconstituted Soluble CD14 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚°C. As with any protein, exposing Soluble CD14 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Form/Appearance	Lyophilized
Disclaimer	For research use only

Alternative Names

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Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

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Quick Database Links

Gene Symbol

CD14

UniProt

P08571

RefSeq (Protein):NP_000582.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000582.1

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