SPECC1 Antibody (Center)

SKU: orb1937711

Description

Research Area

Cell Biology

Images & Validation

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Item 1 of 5

Tested Applications	FC, IF, IHC-P, WB
Dilution Range	WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50, IF - 1:10-50
Reactivity	Human, Mouse
Predicted Reactivity	Gallus, Zebrafish

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	124602 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CYTSA, KIAA0376

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Confocal immunofluorescent analysis of SPECC1 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

SPECC1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

SPECC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SPECC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

SPECC1 Antibody (Center) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).

SPECC1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane). This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).

Quick Database Links

UniProt

Q69YQ0

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

Flow Cytometry

Immunofluorescence