Spectrin beta III Antibody / SPTBN2

SKU: orb2640225

Description

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Spectrin alpha and spectrin beta are present in erythrocytes, whereas spectrin alpha II (fodrin a) and spectrin beta I (fodrin b) are present in other somatic cells. SPTBN2/Spectrin beta III is highly homologous to both spectrin beta I and spectrin beta II. SPTBN2 is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.

Research Area

Neuroscience

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution Range	Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Reactivity	Human, Mouse, Rat
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Spectrin beta III antibody to be titered up or down for optimal performance.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	SPTBN2/1584
Immunogen	Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this Spectrin beta III antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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