Spectrin beta III Antibody / SPTBN2

SKU: orb750058

Description

Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Spectrin alpha and spectrin beta are present in erythrocytes, whereas spectrin alpha II (fodrin a) and spectrin beta I (fodrin b) are present in other somatic cells. SPTBN2/Spectrin beta III is highly homologous to both spectrin beta I and spectrin beta II. SPTBN2 is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.

Research Area

Epigenetics, Neuroscience

Images & Validation

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Item 1 of 9

Tested Applications	FACS, IF, IHC-P, WB
Dilution Range	Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Reactivity	Human, Mouse, Rat
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Spectrin beta III antibody to be titered up or down for optimal performance.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	SPTBN2/1584
Immunogen	Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this Spectrin beta III antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Western blot testing of human PANC-1 cell lysate with Spectrin beta III antibody. Predicted molecular weight ~246 kDa.

Western blot testing of human HeLa cell lysate with Spectrin beta III antibody. Predicted molecular weight ~246 kDa.

IHC testing of FFPE human pancreas with Spectrin beta III antibody (clone SPTBN2/1584). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE mouse kidney with Spectrin beta III antibody (clone SPTBN2/1584). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE rat pancreas with Spectrin beta III antibody (clone SPTBN2/1584). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of MeOH fixed human HeLa cells with Spectrin beta III antibody (clone SPTBN2/1584, green) and Reddot nuclear stain (red).

Flow cytometry staining of human HeLa cells with Spectrin beta III antibody; Red = isotype control, Blue = Spectrin beta III antibody.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Spectrin beta III antibody (clone SPTBN2/1584). These results demonstrate the foremost specificity of the SPTBN2/1584 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE Analysis of Purified, BSA-Free Spectrin beta III Antibody (clone SPTBN2/1584). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

O15020

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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