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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:2000, IHC-P - 1:25 |
| Reactivity | Human, Monkey, Mouse |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1,k |
| Clone No. | B3905EV758X485X41 |
| Immunogen | This SUFU antibody is generated from a mouse immunized with a recombinant protein of human SUFU. |
| Target | SUFU {ECO:0000303|PubMed:12068298, ECO:0000312|HGNC:HGNC:16466} |
| Molecular Weight | 53947 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Suppressor of fused homolog, SUFUH, SUFU
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All lanes: Anti-SUFU Antibody at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: COS-7 whole cell lysate. Lane 3: LNCap whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Staining SUFU in mouse embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Quick Database Links
Gene Symbol
SUFU {ECO:0000303|PubMed:12068298, ECO:0000312|HGNC:HGNC:16466}
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
SUFU Antibody (orb1925587)
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