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SUMO1 Antibody (C-term)
SKU: orb1937645
Description
Research Area
Cardiovascular Research, Cell Biology, Infectious Disease & Virology, Metabolism Research
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50 |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Mouse, Porcine, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 11557 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SMT3C, SMT3H3, UBL1
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COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels). Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels: cell lysates probed by western blot (WB) with an anti-ERM Antibody. Center panels: cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by WB with SUMO-1 Antibody. Bottom panels: cell lysates immunoprecipitated with an anti-FLAG antibody followed by WB with SUMO-2/3 Antibody. (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
SUMO1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
The anti-SUMO1 polyclonal antibody is used in Western blot to detect GST-SUMO1 fusion protein.
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
SUMO1 Antibody (C-term) (orb1937645)
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