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SUMO1 Antibody (N-term E67)
SKU: orb30213
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution range | IF/ICC: 1:10-50, WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB3373 |
| Immunogen | Synthetic Peptide |
| Target | SUMO1 |
| Molecular Weight | 11557 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−Anti-Small ubiquitin-related modifier 1 antibody, anti-SUMO-1 antibody, anti-Ubiquitin-like protein SMT3C antibody, anti-SMT3 homolog 3 antibody, anti-Ubiquitin-homology domain protein PIC1 antibody, anti-Ubiquitin-like protein UBL1 antibody, anti-GAP modifying protein 1 antibody, anti-GMP1 antibody, anti-Sentrin antibody, anti-SMT3C antibody, anti-SMT3H3 antibody, anti-UBL1 antibody
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Fluorescent image of Hela cell stained with SUMO1 Antibody (N-term E67). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SUMO1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). SUMO1 immunoreactivity is localized to Nucleus significantly.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
SUMO1 Antibody (N-term E67) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of anti-SUMO1 polyclonal antibody in A375 cell line lysate. SUMO1 (arrow) was detected using the purified Pab.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_003343.1, NP_001005782.1, NP_001005781.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
SUMO1 Antibody (N-term E67) (orb30213)
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