SUMO2 Antibody (C-term)

SKU: orb1937154

Description

Images & Validation

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Item 1 of 4

Tested Applications	IHC-P, WB
Dilution range	WB - 1:1000, IHC-P - 1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Gallus, Hamster, Monkey, Porcine, Xenopus, Zebrafish

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	10871 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Disclaimer	For research use only

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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The SUMO2 C-term Antibody is used in Western blot to detect SUMO2 in GST-SUMO2 fusion protein (lane 1) and HL60 cell lysate (lane 2).

Western blot analysis of lysates from 293T, HL-60, Jurkat cell line (from left to right), using SUMO2 Antibody. diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary Antibody. Lysates at 35 ug per lane.

Western blot analysis of SUMO2 (arrow) using rabbit polyclonal SUMO2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2 gene.

Quick Database Links

UniProt

P61956

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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