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SUMO2/3 Antibody (N-term)
SKU: orb1937637
Description
Images & Validation
−Item 1 of 3
| Tested Applications | WB |
|---|---|
| Dilution range | WB - 1:2000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bacteria, Bovine, C. elegans, Drosophila, Gallus, Hamster, Monkey, Porcine, Rabbit, Virus, Xenopus, Yeast, Zebrafish |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 10871 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Disclaimer | For research use only |
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All lanes: Anti-SUMO2/3 Antibody (M1) at 1:2000 dilution. Lane 1: A549 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: HepG2 whole cell lysates. Lane 4: HL-60 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 11 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of anti-SUMO2/3 N-term polyclonal antibody in 293 cell lysate. Sumo2/3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of SUMO2/3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (M1). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2/3 gene.
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SUMO2/3 Antibody (N-term) (orb1937637)
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