Recombinant Synaptophysin Antibody

SKU: orb699710

Description

This recombinant rabbit monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.

Research Area

Epigenetics

Images & Validation

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Tested Applications	IHC-P
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Synaptophysin antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG
Clone No.	SYP/4503R
Immunogen	A portion of amino acids 224-313 from the human protein was used as the immunogen for the recombinant Synaptophysin antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human pancreas with recombinant Synaptophysin antibody (clone SYP/4503R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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UniProt

P08247

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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