Syndecan-1 Recombinant Protein

SKU: orb1231484

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Tested Applications	ELISA, WB
Application Notes	This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50.3 kDa. The protein migrates as 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Source	HEK293 cells
Tag	Fc Tag
Molecular Weight	50.3 kDa
Protein Sequence	Gln 23 - Gly 254
Purity	>95% as determined by SDS-PAGE.

Storage & Handling

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Storage	Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	PBS, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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SDC1,Syndecan-1,CD138,SYND1,SDC

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Human Syndecan-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized Human Syndecan-1, Fc Tag at 1 ug/ml (100 uL/well) can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.1-3 ng/mL.

Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 ug/ml (100 uL/well) can bind Human Syndecan-1, Fc Tag with a linear range of 1-2 ng/mL.

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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