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Syndecan-1 Recombinant Protein
SKU: orb1231484
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Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Tag | Fc Tag |
| Molecular Weight | 50.3 kDa |
| Protein Sequence | Gln 23 - Gly 254 |
| Purity | >95% as determined by SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Disclaimer | For research use only |
Alternative Names
−SDC1,Syndecan-1,CD138,SYND1,SDC
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Human CD138 Protein, hFc-His Tag [orb689367]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 50.8 kDa after removal of the signal peptide. The apparent molecular mass of CD138-hFc-His is approximately 70-100 kDa due to glycosylation.
Mammalian
50 μg, 10 μg, 100 μg
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Human Syndecan-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human Syndecan-1, Fc Tag at 1 ug/ml (100 uL/well) can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.1-3 ng/mL.
Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 ug/ml (100 uL/well) can bind Human Syndecan-1, Fc Tag with a linear range of 1-2 ng/mL.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Syndecan-1 Recombinant Protein (orb1231484)
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