Talacotuzumab Antibody

SKU: orb1980561

Description

Talacotuzumab is a humanized anti-CD123 IgG1 monoclonal antibody engineered for enhanced ADCC via Fc modification. It blocks IL-3 signaling and demonstrates potent anti-leukemic activity, supporting its research in acute myeloid leukemia (AML) models both in vitro and in vivo.

Research Area

Immunology & Inflammation

Images & Validation

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Key Properties

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CAS Number	1826831-79-1
MW	147.62 kDa
Purity	98.42% (SEC-HPLC)
Target	Immunology/Inflammation related,Interleukin

Bioactivity

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Target IC50	TF-1 cells:33 pM
In Vivo	In mice with xenografts of acute myeloid leukemia,Talacotuzumab (JNJ 56022473; CSL 362; 300 μg; intraperitoneal injection; three times per week for 5 weeks) significantly delays tumor growth compared to the isotype control . Talacotuzumab (1, 10, 30 mg/kg; subcutaneous injection; single dose) in juvenile cynomolgus monkeys reaches maximum serum concentrations of 12, 190, and 380 μg/ml at doses of 1, 10, and 30 mg/kg, respectively, within 48 hours . Read more...
In Vitro	With an IC50 of 5 ng/ml (33 pM), Talacotuzumab (JNJ 56022473; CSL 362) potently mediates antibody-dependent cell-mediated cytotoxicity (ADCC) in TF-1 cells . Following a 24-hour pretreatment at 1 μg/ml,Talacotuzumab inhibits the production of IFN-α in plasmacytoid dendritic cells and eosinophils in SLE (systemic lupus erythematosus) donor and healthy donor plasma-like dendritic cells. This inhibition occurs when these cells are stimulated with TLR7 (imiquimod; 0.5 μM; 6 days) and TLR9 (CpG C; 0.5 μM; 6 days). However, Talacotuzumab does not significantly reduce IFN-α production induced by TLR4 stimulation (LPS; 10 μg/ml). The inhibitory effect of Talacotuzumab on plasmacytoid dendritic cell (pDC) proliferation and expansion induced by TLR7 and TLR9 is achieved through the depletion of pDC . Read more...

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD123, CSL362, CSL-362, CSL 362, Interleukin Related, JNJ 56022473, JNJ56022473, JNJ-56022473, Inflammation related, Inflammationrelated, Immunologyrelated, Immunology related, Talacotuzumab

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Gene Symbol

Immunology/Inflammation related,Interleukin

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