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TbHsp70 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Dilution range | WB (1:1000) |
| Reactivity | Plant |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide from the mid-protein of Trypanosoma brucei brucei TbHsp70 (aa. 200-300) |
| Target | TbHsp70 |
| Molecular Weight | 71 kDa |
| Purification | Peptide Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of Trypanosoma brucei brucei cells showing detection of ~71 kDa TbHsp70 protein using Rabbit Anti-TbHsp70 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cells incubated for 1 hour at 37°C. Lane 3: Cells incubated for 1 hour at 42°C. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TbHsp70 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71 kDa.
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TbHsp70 Antibody (orb535190)
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