TGFB2 Antibody / TGF beta 2

SKU: orb1824171

Description

Transforming growth factor Bs (TGFbs) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFa. It is now realized that TGFbs mediate many cell-cell interactions that occur during embryonic development. Three TGFbs have been identified in mammals. TGFb1, TGFb2 and TGFb3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGFb requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGFb3 protein has approximately 80% identity to the mature region of both TGFb1 and TGFb2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity.

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Tested Applications	IHC-P
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	TGFB2/1679
Immunogen	Recombinant full-length human protein was used as the immunogen for the TGF beta 2 antibody.

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human prostate carcinoma tissue with TGF beta 2 antibody (clone TGFB2/1679). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free TGF beta 2 antibody (clone TGFB2/1679) as confirmation of integrity and purity.

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UniProt

P10600

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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