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TIGIT Antibody
SKU: orb1274207
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 6
| Tested Applications | ELISA, FC, ICC, IF, IHC-P |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Camelus |
| Clonality | Recombinant |
| Isotype | sdAb |
| Clone No. | 2D7 |
| Immunogen | TIGIT antibody was raised against a recombinant protein corresponding to amino acids 22-141 of human TIGIT. |
| Target | TIGIT |
| Purification | TIGIT Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | TIGIT Antibody is supplied in PBS. |
| Concentration | 1 mg/mL |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−TIGIT Antibody: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
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Immunocytochemistry of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 10 μg/mL.
Immunofluorescence of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 20 μg/mL.
Immunofluorescence of TIGIT in human spleen tissue with TIGIT single domain antibody at 20 μg/mL.
Titration ELISA analysis of TIGIT sdAbs to detect recombinant TIGIT (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.
Immunohistochemistry of TIGIT in human spleen tissue with TIGIT single domain antibody at 1 μg/mL.
Flow cytometry analysis of TIGIT overexpressing HEK293 cells using TIGIT single domain antibody and control 1H4 single domain antibody at 0.05 μg/ml. Blue: Untransfected HEK293 cells. Yellow: TIGIT overexpressing HEK293 cells.
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
TIGIT Antibody (orb1274207)
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