Tnf Antibody

SKU: orb420300

This product can be custom made

Description

TNF alpha antibody

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution Range	ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000
Reactivity	Mouse
Application Notes	This antibody against Mouse TNFα has been tested for use in immunoblotting. This antibody is suitable for ELISA, immunohistochemistry, neutralizations, radioimmunoassay, and immunoprecipitation. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown. This antibody will recognize the cell-bound precursor of TNFα as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of mouse activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	This antibody was produced from whole rabbit serum prepared by repeated immunizations with recombinant mouse TNFα produced in E.coli.
Target	Tnf
Purity	TNF alpha Antibody has been heated to 56°C for 30 minutes. The antibody is directed against mature 17,000 MW mouse TNFα and is useful in determining its presence in various assays. The antibody does not recognize mouse TNFß (lymphotoxin).
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/ml
Expiration Date	12 months from date of receipt.
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

Alternative Names

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rabbit anti-TNF alpha antibody, rabbit anti-TNFa antibody, rabbit anti-tumor necrosis factor alpha antibody, APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNFA

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western Blot of Mouse TNFα Antibody. Lane 1: Mouse TNFα. Load: 50 ng per lane. Primary antibody: Mouse TNFα antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Mouse TNFα.

Quick Database Links

Gene Symbol

Tnf

UniProt

P06804

RefSeq:P06804.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	P06804.2

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Datasheet

Product Information

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Tnf Antibody (orb420300)

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Applications:	ELISA, FC, IHC, WB
Reactivity:	Human, Monkey, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, ICC, IF, IHC-P, WB
Reactivity:	Human, Porcine, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	FC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Canine, Rat
Reactivity:	Human, Mouse
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	IF, IHC-Fr, IHC-P
Predicted Reactivity:	Mouse, Rat
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Recombinant
Conjugation:	Unconjugated

Applications:	ICC, IF, IHC-P
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Tnf Antibody

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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Quality Guarantee

Quick Database Links

UniProt Details

NCBI Reference Sequences

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Protocol Information

Tnf Antibody (orb420300)

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