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TNF alpha Mouse Monoclonal Antibody

SKU: orb632005

Description

Mouse monoclonal antibody to TNF alpha

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeWB: 1:500-1:5000; IHC: 1:200-1:1000
ReactivityHuman, Mouse, Rat

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG2b
Immunogentumor necrosis factor(TNF superfamily, member 2)
TargetTNF alpha
Purity≥95% as determined by SDS-PAGE
PurificationProtein A+G purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearanceliquid
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNFSF2, Tumor necrosis factor

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

TNF alpha Mouse Monoclonal Antibody

LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with orb632005 (TNF-a Antibody) at dilution of 1:1000.

TNF alpha Mouse Monoclonal Antibody

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using orb632005 (TNF-a Antibody) at dilution of 1:800. heat mediated antigen retrieved with Tris-EDTA buffer (pH9).

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

TNF alpha Mouse Monoclonal Antibody (orb632005)

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Available Sizes

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50 μg
¥ 3,120.00
100 μg
¥ 5,330.00
Choose Conjugation or Carrier Free Version
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