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Description
Research Area
Immunology & Inflammation
Images & Validation
−
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:1,000 - 1:5,000, IHC: 1:100 - 1:500, WB: 1:500 - 1:2,000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Antiserum |
| Immunogen | The whole rabbit serum was prepared by repeated immunizations with recombinant mouse TNFa produced in E.coli. |
| Target | Tnf |
| Purity | This antiserum has been heated to 56°C for 30 minutes. The antiserum is directed against mature 17,000 MW mouse TNFa and is useful in determining its presence in various assays. The antibody does not recognize mouse TNFb (lymphotoxin). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | None |
| Concentration | 85 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Mouse TNF alpha Antibody, APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Tnf Antibody (orb750714)
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