TNF alpha Antibody

SKU: orb2641896

Description

Tumor Necrosis Factor Alpha (TNF alpha) is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. [UniProt]

Images & Validation

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Tested Applications	FACS, IF, IHC-P
Dilution range	Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
Reactivity	Human, Rat
Application Notes	Titering of the TNF alpha antibody may be required for optimal performance.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgM, kappa
Clone No.	TMDa1-1
Immunogen	Full length human recombinant protein was used as the immunogen for the TNF alpha antibody.
Purification	PEG precipitation
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Immunofluorescent staining of PFA-fixed human HepG2 cells with TNF-alpha antibody (green, clone TMDa1-1) and Reddot nuclear stain (red).

IHC testing of FFPE human histiocytoma and TNF alpha antibody (clone TMDa1-1). Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE rat stomach and TNF alpha antibody (clone TMDa1-1). Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry testing of PFA-fixed human HepG2 cells with TNF-alpha antibody (clone TMDa1-1); Red = isotype control, Blue = TNF-alpha antibody.

Quick Database Links

UniProt

P01375

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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