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Description
Research Area
Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Canine, Equine, Mouse, Porcine |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human TNF-alpha (181-235/233aa) |
| Target | TNF |
| Molecular Weight | 17/26 kDa |
| Purification | affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tumor necrosis factor-α; TNFalpha; APC1; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; MCF; Necrosin; TNF a; TNF-alpha; TNF; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precurso; Tumour Necrosis Factor alpha; TNFA_HUMAN.
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Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-TNF alpha (orb100003) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 26/17 kD, Observed band size: 26 kD.
Tissue/cell: human pneumonia tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-TNF-alpha Polyclonal Antibody, Unconjugated (orb100003) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
Gene Symbol
TNF
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
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Dong-qing Liu, Xiao-jing Li, Xiao-juan Weng Effect of BTXA on Inhibiting Hypertrophic Scar Formation in a Rabbit Ear Model Aesthetic Plastic Surgery, 41, 721 (2017)
Applications
IHC
Reactivity
Rabbit
TNF alpha Rabbit Polyclonal Antibody (orb100003)
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