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TNF-R2 rabbit pAb
SKU: orb766497
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TNF Receptor II. AA range:376-425 |
| Molecular Weight | 48 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-TNFRSF1B antibody, anti-TNFBR antibody, anti-TNFR2 antibody, anti-Tumor necrosis factor receptor superfamily member 1B antibody, anti-Tumor necrosis factor receptor 2 antibody, anti-TNF-R2 antibody, anti-Tumor necrosis factor receptor type II antibody, anti-TNF-RII antibody, anti-TNFR-II antibody, anti-p75 antibody, anti-p80 TNF-alpha rec antibody
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Western Blot analysis of various cells using TNF-R2 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.
Immunofluorescence analysis of HeLa cells, using TNF Receptor II Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from RAW264.7, HT-29, and LOVO cells, using TNF Receptor II Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HUVECcells using TNF Receptor II antibody.
Quick Database Links
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
TNF-R2 rabbit pAb (orb766497)
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