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Description
Research Area
Immunology & Inflammation
Images & Validation
−
| Tested Applications | FC, Functional Studies, IA, IF, IHC-Fr, IP, WB |
|---|---|
| Dilution Range | For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Advised positive controls for frozen sections are human lymphnodes and for flow cytometry PHA activated T cells. |
| Reactivity | Human |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Isotype | Mouse IgG1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin. |
| Buffer/Preservatives | Quantities >500 µg will be sent without preservatives and are carrier-free. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
IP
Immunoprecipitation
TNF-RII, Human, mAb MR2-1 Antibody (orb1109061)
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