TNFRSF6B Antibody (N-term)

SKU: orb1939148

Description

Research Area

Cell Biology, Immunology & Inflammation

Images & Validation

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Item 1 of 4

Tested Applications	FC, IHC-P, WB
Dilution range	WB - 1:2000
Reactivity	Human

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	32680 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Disclaimer	For research use only

Alternative Names

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DCR3, TR6

Quality Guarantee

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All lanes: Anti-TNFRSF6B Antibody (N-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HCT116 whole cell lysate. Lane 3: PC-3 whole cell lysate. Lane 4: SW480 whole cell lysate. Lane 5: HUVEC whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 33 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

TNFRSF6B Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control (rabbit IgG alone) (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

TNFRSF6B antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TNFRSF6B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

TNFRSF6B Antibody (N-term) western blot analysis in 293 cell line lysates (35 ug/lane). This demonstrates the TNFRSF6B antibody detected the TNFRSF6B protein (arrow).

Quick Database Links

UniProt

O95407

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Flow Cytometry

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