TRADD Antibody (Center)

SKU: orb1937832

Description

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	FC, IF, IHC-P, WB
Dilution range	WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50, IF - 1:10-50
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	34247 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Disclaimer	For research use only

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Confocal immunofluorescent analysis of TRADD Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

TRADD Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

TRADD Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TRADD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

TRADD Antibody (Center) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the TRADD antibody detected the TRADD protein (arrow).

Quick Database Links

UniProt

Q15628

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

Flow Cytometry

Immunofluorescence