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TRAF1 Antibody

SKU: orb1246843

Description

TRAF1 Antibody

Research Area

Cell Biology

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityMouse
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50-55kDa band observed in lysates of monocytic leukemia cell line U937 (calculated MW of 46.2kDa according to NP_005649.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pryhuber et al , Am J Respir Cell Mol Biol. 2000 Feb;22(2):150-6. PMID: 10657935). Recommended concentration: 0.3-1.0µg/mL.Immunohistochemistry:Paraffin embedded Human Thymus and Small Intestine. Recommended concentration: 2.5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-KLQSPKHAYVKDD
TargetTRAF1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

TRAF1, TNF receptor-associated factor 1 , EBI6, MGC:10353 , Epstein-Bar virus-induced protein 6

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TRAF1 Antibody

orb1246843 (0.5 ug/ml) staining of U937 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TRAF1 Antibody

orb1246843 (2.5 ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

TRAF1 Antibody

orb1246843 (2.5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

TRAF1 Antibody (orb1246843)

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0.1 mg
¥ 11,960.00
Free Secondary Antibody (20 ul)0/0

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