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TRAIL Recombinant Protein
Description
Research Area
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Biological activity was determined by its ability to induce apoptotic cell death in TRAIL - sensitive U343MG cells. The expected ED50 for this effect is 1.0 - 3.0 ng/mL. |
| Target | TNFSF10 |
| Protein Sequence | MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
Storage & Handling
−| Storage | The lyophilized TRAIL recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted TRAIL is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing TRAIL recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 18 KDa. Observed: 22-30 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human TRAIL R2 Protein (Biotin) [orb2993682]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 17.1 KDa. Observed: 18-25 KDa, reducing conditions
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 23.7 KDa. Observed: 19-30 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human TRAIL R2 Protein [orb2993994]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 42.2 KDa. Observed: 49 KDa, reducing conditions
10 μg, 50 μg, 1 mg, 500 μg

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Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_001177871.1 |
|---|
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Protocol Information
TRAIL Recombinant Protein (orb1230812)
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