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Description
Research Area
Cardiovascular Research, Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | IF - 1:25, WB - 1:2000 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | BTD77199 |
| Immunogen | This Transferrin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 101-135 amino acids from human Transferrin. |
| Target | TF (HGNC:11740) |
| Molecular Weight | 77064 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin, TF
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All lanes: Anti-Transferrin Antibody at 1:2000 dilution. Lane 1: human plasma lysate. Lane 2: human fetal liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 77kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-Transferrin Antibody at 1:2000 dilution. Lane 1: human plasma lysate. Lane 2: human liver lysate. Lane 3: human fetal liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 77 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Transferrin at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).
Quick Database Links
Gene Symbol
TF (HGNC:11740)
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
Transferrin Antibody (orb1925719)
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