TRAP1 Antibody (APC)

SKU: orb181864

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Description

Mouse monoclonal antibody against Trap1, conjugated to APC.

Research Area

Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

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Tested Applications	ICC, IF, WB
Dilution range	WB (1:1000), ICC/IF (1:100)
Reactivity	Human, Mouse, Rat
Application Notes	1 µg/ml of SMC-207 was sufficient for detection of Trap-1/HSP75 in 20 µg of Human A431 lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1 Kappa
Clone No.	3H4-2H6
Immunogen	Purified recombinant Human TRAP1
Target	TRAP1
Molecular Weight	75kDa
Purification	Protein G Purified
Conjugation	APC

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	95.46mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Heat shock protein 75, Heat shock protein 75 kDa, Heat shock protein 75 kDa mitochondrial, HSP 75, HSP 90L, HSP75, HSP90L, mitochondrial, TNF receptor associated protein 1, TNFR associated protein 1, TNFR-associated protein 1, TRAP 1, TRAP-1, Trap1, TRAP1_HUMAN, Tumor necrosis factor receptor associated protein, Tumor necrosis factor type 1 receptor associated protein, Tumor necrosis factor type 1 receptor-associated protein

Quality Guarantee

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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Trap1 Monoclonal Antibody, Clone 3H4-2H6. Tissue: Myoblast cell line C2C12. Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Western Blot analysis of Human, Rat Human A431 and Rat Brain Membrane cell lysates showing detection of ~75 kDa Trap1 protein using Mouse Anti-Trap1 Monoclonal Antibody, Clone 3H4-2H6. Lane 1: MW ladder. Lane 2: Human lysate, A431. Lane 3: Rat lysate, Rat Brain Membrane (RBM). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Color Development: TMB solution for 15 min at RT. Predicted/Observed Size: ~75 kDa.

Quick Database Links

Gene Symbol

TRAP1

UniProt

Q12931

NCBI Gene ID

10131

RefSeq (Protein):NP_057376.2

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_057376.2

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ICC

Immunocytochemistry

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