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HIST1H3A Antibody
SKU: orb685608
Description
Research Area
Epigenetics & Chromatin
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | WB: 1: 500-1: 1000, IHC-P: 1: 50-1: 100, IF: 1: 100-1: 200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Peptide sequence around Tri-Methylation site of lysine 27(A-R-K(tri-methyl)-S-A) derived from Human Histone H3. |
| Target | HIST1H3A |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy u |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Buffer/Preservatives | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Disclaimer | For research use only |
Alternative Names
−Anti-H3/a antibody, anti- H3/c antibody, anti- H3/d antibody, anti- H3/f antibody, anti- H3/h antibody
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Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Western blot analysis of HT29 and C6 cells using Tri-Methyl-Histone H3 (Lys27) antibody
Immunohistochemical staining of human breast carcinoma tissue using Tri-Methyl-Histone H3 (Lys27) antibody
Immunofluorescence analysis of MEF cells using Tri-Methyl-Histone H3 (Lys27) antibody
Quick Database Links
Gene Symbol
HIST1H3A
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
HIST1H3A Antibody (orb685608)
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