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H3K27me3 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ChIP, IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
| Target | H3K27me3 |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
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50 μl, 100 μl, 200 μlAnti-H3K27me3 [BT164] [orb758979]
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0.2 mgAnti-H3K27me3 [BT164] [orb758980]
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0.2 mgHistone H3 K79me3 Antibody [orb420422]
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Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (orb1259103) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody (orb1259103) at dilution of 1:25 (40x lens).

Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (orb1259103) at dilution of 1:25. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 antibody (orb1259103) at dilution of 1:25. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2OS cells using TriMethyl-Histone H3-K27 antibody (orb1259103) at dilution of 1:25. Blue: DAPI for nuclear staining.
Quick Database Links
UniProt Details
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Protocol Information
H3K27me3 Antibody (orb1259103)
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