H3K27me3 Antibody

SKU: orb1258185

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Description

H3K27me3 Antibody

Research Area

Signal Transduction

Images & Validation

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Item 1 of 9

Tested Applications	ChIP, IF, IHC, IP, WB
Reactivity	Human, Mouse, Other, Rat
Application Notes	WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
Target	H3K27me3
Molecular Weight	Observed: 17kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentration	batch dependent
Disclaimer	For research use only

Alternative Names

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H3F3A, H3t, H3.4, H3/g, H3FT

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Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (orb1258185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (orb1258185).

Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K27 antibody (orb1258185). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (Red) (orb1258185) and BrdU antibody (Green) (A1482). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

H3K27me3

UniProt

Q16695

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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Immunoprecipitation

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ChIP

Chromatin Immunoprecipitation

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