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TriMethyl-Histone H3 (Lys27) Rabbit mAb
SKU: orb1564023
Featured
Description
Research Area
Epigenetics & Chromatin
Images & Validation
−Item 1 of 3
| Tested Applications | ChIP, ICC, IF, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ChIP: 1/20 |
| Reactivity | Human, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | R01-8I4 |
| Immunogen | A synthetic methyl-peptide corresponding to residues surrounding Lys27 of human Histone H3 |
| Target | H3C1 |
| Molecular Weight | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin. |
| Concentration | 0.3 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−H3K27me3; H3 histone; HIST1H3A; Histone cluster 1; H3a
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Western blot analysis of Histone H3 (tri methylLys27) in C6, Jurkat lysates using TriMethyl-Histone H3 (Lys27) antibody.
Immunocytochemistry analysis of TriMethyl-Histone H3 (Lys27) (green) in Hela using TriMethyl-Histone H3 (Lys27) antibody, and DAPI (blue).
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using TriMethyl-Histone H3 (Lys27) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Quick Database Links
Gene Symbol
H3C1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
IP
Immunoprecipitation
ChIP
Chromatin Immunoprecipitation
TriMethyl-Histone H3 (Lys27) Rabbit mAb (orb1564023)
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