Celastrol

SKU: orb1304608

Featured

Description

Tripterin

Research Area

Cell Biology, Epigenetics & Chromatin, Neuroscience, Protein Biochemistry

Images & Validation

−

Key Properties

−

CAS Number	34157-83-0
MW	450.62
Purity	>98%
Formula	C₂₉H₃₈O₄
SMILES	CC1=C(O)C(=O)C=C2C1=CC=C1[C@@]2(C)CC[C@@]2(C)[C@@H]3C[C@@](C)(CC[C@]3(C)CC[C@]12C)C(O)=O
Target	Proteasome
Solubility	Soluble in DMSO (up to 45 mg/ml) or in Ethanol (up to 30 mg/ml).

Bioactivity

−

Target IC50	1A9 cells:0.091 μg/mL (ED50)\|A172 cells:2.31 μM\|20S proteasome:2.5 μM
In Vivo	METHODS: To detect anti-tumor activity in vivo, Tripterin (1-3 mg/kg, 10% DMSO+70% Cremophor/ethanol (3:1)+20% PBS) was injected intraperitoneally once daily for sixteen days into nude immunodeficient mice bearing human prostate cancer tumor PC-3. RESULTS: Tripterin treatment significantly inhibited the growth of prostate cancer xenografts and suppressed proteasome activity and induced apoptosis in vivo. METHODS: To detect anti-tumor activity in vivo, Tripterin (1.25 mg/kg) was intraperitoneally injected into BALB/c (nu/nu) mice bearing vestibular nerve sheath tumor SC4 every three days for two weeks. RESULTS: Tripterin significantly inhibited tumor growth without showing toxicity. Read more...
In Vitro	METHODS: Human prostate cancer cells PC-3 were treated with Tripterin (0.5-5 µM) for 12 h. Proteasomal chymotrypsin-like activity was assayed using Z-GGL-AMC. RESULTS: Tripterin significantly inhibited proteasomal chymotrypsin-like activity in PC-3 cells in a concentration-dependent manner, reaching about 55% inhibition at 2.5 µM. METHODS: Human chronic myeloid leukemia cells KBM-5 were incubated with Tripterin (2.5 µM) for 6 h, followed by treatment with TNF (1 nM) for 6-24 h. Target protein expression levels were detected using Western Blot. RESULTS: TNF induced the expression of anti-apoptotic proteins IAP1, IAP2, Bcl-2, Bcl-XL, c-FLIP and survivin in a time-dependent manner, which was inhibited by Tripterin. Read more...
Cell Research	The anti-proliferative effect of celastrol on various human tumor cell lines is determined by the MTT uptake method. Briefly, 5×103 cells are incubated with Celastrol in triplicate in a 96-well plate at 37 ℃. MTT solution is then added to each well. After a 2 hours incubation at 37 ℃, extraction buffer (20% SDS, 50% dimethylformamide) is added, cells are incubated overnight at 37 ℃, and the optical density is then measured at 570 nm using a Tecan plate reader.(Only for Reference) Read more...

Storage & Handling

−

Storage	-20°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Tripterin, Tripterine, Proteasome, inhibit, Mitochondrial Autophagy, Mitophagy, Endogenous Metabolite, Inhibitor, 20S proteasome, Autophagy, Apoptosis, Celastrol

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Compound Identifiers

PubChem CID

122724 ↗

Key Properties

−

No computed properties available.

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols

Celastrol (orb1304608)

Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

No reviews yet

CAS Number:	1258-84-0
MW:	464.64
Formula:	C₃₀H₄₀O₄

CAS Number:	34157-83-0
MW:	450.61
Formula:	C₂₉H₃₈O₄

CAS Number:	2413849-81-5
MW:	681.83
Formula:	C₄₂H₄₈FNO₆

CAS Number:	2027514-32-3
MW:	557.77
Formula:	C₃₅H₄₇N₃O₃

CAS Number:	3033049-16-7
MW:	559.8
Formula:	C₃₅H₄₅NO₃S

Celastrol

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

Similar Products

Pristimerin (Standard) [orb3139500]

Tripterin (Standard) [orb3140252]

CAY10747 [orb1693951]

LC-PDin06 [orb3135379]

STAT3-IN-38 [orb2814277]

Quality Guarantee

Compound Identifiers

Key Properties

Documents Download

Request a Document

Protocol Information

Celastrol (orb1304608)

Step 1: Enter information below

Step 2: Enter the in vivo formulation

Available Sizes

Email

UK Office

US Office

CN Office

Distributors