Trypsin Inhibitor Antibody Peroxidase Conjugated

SKU: orb344907

Description

Trypsin Inhibitor antibody (Peroxidase)

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:1,000 - 1:5,000
Reactivity	Plant
Application Notes	Anti-Trypsin Inhibitor peroxidase conjugated antibody has been tested by western blotting and ELISA and is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-Trypsin Inhibitor was produced by repeated immunizations with soy bean trypsin inhibitor.
Purity	Anti-Trypsin Inhibitor antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor (Soy Bean). Cross-reactivity against Trypsin Inhibitor from other tissues and species is unknown.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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rabbit anti-trypsin inhibitor antibody HRP conjugation, peroxidase conjugated rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

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Trypsin Inhibitor Antibody Peroxidase Conjugated

Western Blot of Rabbit Anti Trypsin Inhibitor antibody. Lane 1: purified Soybean Trypsin Inhibitor Reduced. Lane 2: purified Soybean Trypsin Inhibitor Non-Reduced. Lane 3: purified Streptavidin Reduced. Lane 4: purified Streptavidin Non-Reduced. Load: 1.0 ug per lane. Primary antibody: Biotin conjugated Rabbit anti-trypsin inhibitor antibody and Rabbit anti streptavidin 1:1000 for overnight at 4°C. Secondary antibody: Dylight 649 conjugated Donkey anti rabbit at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 25 kDa for Trypsin Inhibitor.

Quick Database Links

UniProt

P01070

RefSeq:P01070.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	P01070.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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