Trypsin Inhibitor Antibody Peroxidase Conjugated

SKU: orb344915

Description

Trypsin Inhibitor antibody (Peroxidase)

Images & Validation

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Tested Applications	DOT, IP, WB
Dilution range	IP: 1:100, WB: 1:250
Reactivity	Plant
Application Notes	Anti-Trypsin Inhibitor Peroxidase Conjugated Antibody has been tested by Dot blot and is suitable to be assayed against 1.0 ug of Trypsin Inhibitor [Lima Bean] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Trypsin Inhibitor [Lima Bean]
Purity	Trypsin Inhibitor is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean]. Cross reactivity against Trypsin Inhibitor from other tissues and species may occur but have not been specifically determined.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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rabbit anti-trypsin inhibitor antibody HRP conjugation, peroxidase conjugated rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

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Dot Blot Results of Rabbit Anti-Trypsin Inhibitor Antibody Peroxidase Conjugated. Trypsin Inhibitor (1) 100 ng, (2) 33.33 ng, (3) 11.11 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: Anti-Trypsin Inhibitor HRP at 1.0 µg/ml for 1hr at RT. Secondary Antibody: none. Blocking Buffer: BlockOut Buffer (p/n orb348644) for 30 mins at RT. Exposure: 0.25sec.

Quick Database Links

UniProt

P01070

RefSeq:P01070.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	P01070.2

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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DOT

Dot Blot

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