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Turbo Nuclease

SKU: orb532709

Description

Turbo Nuclease is a broad-spectrum endonuclease that cleaves both DNA and RNA molecules independently of being single-or double-stranded, circular, linear or supercoiled. The enzyme is highly stable and active in a broad range of pH and temperature, making it ideal for a variety of downstream processes that require the degradation of DNA/RNA in a simple, efficient and specific manner. Endonuclease from Serratia macescens recombinant expressed and purified from E.coli. Catalytic activity from pH 6 to 10 (optimal around 8.8) and tem-perature 0 to 44 °C. High catalytic efficiency (34-fold greater than DNase I). Activity requires the presence of Mg2+ (optimum 2 mM).

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Application Notes
Turbo Nuclease is very effective in degrading nucleic acid from pro-tein samples and it is used in a variety of application where complete hydrolysis of DNA/RNA is required: Reduction of viscosity from cell lysates. Prevention of cell clumping. Elimination of unspecific protein-DNA complexes prior 2D-or native gel electrophoresis. Removal of nucleic acids from large-scale protein preparations.

Key Properties

SourceE. coli
Biological OriginSerratia marcescens

Storage & Handling

Storagestore at -20 °C
Form/Appearanceliquid (Supplied in 20 mM Tris-HCl pH 8.0, 20 mM NaCl, 2 mM MgCl 2, 1 mM DTT and 50% [v/v] glycerol)
Concentration250 units/µl
DisclaimerFor research use only

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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Turbo Nuclease (orb532709)

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5 x 10000 units
¥ 8,970.00
DispatchUsually dispatched within 5-10 working days
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