UAP56 Rabbit Polyclonal Antibody

SKU: orb5592

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Description

Research Area

Epigenetics and Nuclear Signaling

Images & Validation

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Item 1 of 3

Tested Applications	FC, IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2ug/test
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse, Porcine, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human BAT1 (101-200/428aa)
Target	DDX39B
Molecular Weight	49 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; B(0,+)-type amino acid transporter 1; BAT1; Bat1a; DEAD box protein UAP56; Glycoprotein-associated amino acid transporter b0,+AT1; HLA-B-associated transcript 1 protein; HLA-B-associated transcript 1A; HLA-B-associated transcript-1; p47; Solute carrier family 7 member 9; Spliceosome RNA helicase BAT1; UAP56; 56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; 0610030D10Rik; 4F2-LC6; AI428441; D17H6S81E; D17H6S81E-1; D6S81E; D6S81Eh; DDX39B; DX39B_HUMAN; Glycoprotein-associated amino acid transporter b0,+AT1; MGC127051; MGC19235; MGC38799; nuclear RNA helicase(DEAD family); OTTHUMP00000029229; OTTHUMP00000165889; OTTHUMP00000165890; Spliceosome RNA helicase DDX39B; U2AF65-associayed protein, 56-KD.

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Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Hyaluronidase3 antibody, dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 0.2 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Tissue/Cell: Human laryngeal tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-UAP56 Polyclonal Antibody, Unconjugated (orb5592) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human laryngeal tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-UAP56 Polyclonal Antibody, Unconjugated (orb5592) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

DDX39B

UniProt

Q13838

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence