VDAC3 Antibody (Center)

SKU: orb140381

This product can be custom made

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Description

Rabbit Polyclonal to VDAC3.

Research Area

Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

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Item 1 of 7

Tested Applications	FC, IHC-P, WB
Dilution Range	WB - 1:1000, IHC-P-Leica - 1:500, IHC-P - 1:100, FC - 1:25
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Porcine, Rabbit

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	RB37017
Immunogen	Synthetic Peptide
Target	VDAC3
Molecular Weight	30659
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-Voltage-dependent anion-selective channel protein 3 antibody, anti-VDAC3 antibody, anti- antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

VDAC3 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the VDAC3 antibody detected the VDAC3 protein (arrow).

Immunohistochemical analysis of paraffin-embedded human testis tissue was performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis on paraffin-embedded Human heart tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis on paraffin-embedded Human testis tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-VDAC3 Antibody (Center) at 1:1000 dilution. Lane 1: Human heart lysate. Lane 2: NCI-H460 whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: U-2OS whole cell lysate. Lane 5: Mouse testis lysate. Lane 6: Rat brain lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 31 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-VDAC3 Antibody (Center) at 1:1000 dilution. Lane 1: Human heart lysate. Lane 2: NCI-H460 whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: U-2OS whole cell lysate. Lane 5: Mouse testis lysate. Lane 6: Mouse heart lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 31 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing Hela cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.