VEGF 121 Recombinant Protein

SKU: orb1227837

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Description

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications	WB
Application Notes	This recombinant protein can be used for WB. For research use only.

Key Properties

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Source	HEK293 cells
Biological Activity	Measured by its binding ability in a functional ELISA. Immobilized ActiveMax Human VEGF121 at 2 ug/mL can bind Human VEGF R2, Strep Tag with a linear range of 0.15-2.5 ug/mL.
Target	VEGFA
Tag	Tag free
Molecular Weight	14 kDa (monomer)
Protein Sequence	Ala 27 - Arg 147
Purity	>95% as determined by SDS-PAGE of reduced (+) and non-reduced (-) rhVEGF121.

Storage & Handling

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Storage	Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	PBS, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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VEGF121, VEGFA, RP1-261G23.1, MGC70609, MVCD1, VEGF, VPF

Recombinant Human VEGF 121 Protein [orb2994931]

Conjugation:	Unconjugated
Purity:	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC.(QC verified)
MW:	Predicted: 15.1 KDa. Observed: 16-18 KDa, reducing conditions

Sizes Available: 10 μg, 50 μg, 500 μg, 1 mg

Item 1 of 1
Recombinant human VEGF-121 protein (Active, HEK293) [orb1817183]
Purity:
>95% as determined by SDS-PAGE
MW:
30-40 kDa
Sizes Available: 10 μg, 50 μg, 500 μg
Item 1 of 1
Recombinant human VEGF-121 protein (HEK293) [orb1808050]
Purity:
>90% as determined by SDS-PAGE
MW:
14.1 kDa
Sizes Available: 20 μg, 100 μg, 500 μg
RecombinantVEGF121,Human [orb1494762]
Purity:
> 95% as analyzed by SDS-PAGE and HPLC.
MW:
28.2 kDa, observed by non-reducing SDS-PAGE.
Source:
Escherichia coli.
Sizes Available: 10 μg, 50 μg

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Human VEGF121, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Quick Database Links

Gene Symbol

VEGFA

UniProt

P15692

RefSeq (Protein):NP_001020541

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001020541

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Protocol Information

Western Blot (IB, immunoblot)

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