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W146

SKU: orb2901680

Description

W146 is a selective antagonist of sphingosine-1-phosphate receptor 1 (S1PR1) with an EC50 value of 398 nM.

Images & Validation

Key Properties

CAS Number909725-61-7
MW342.37
Purity>98% (HPLC)
FormulaC16H27N2O4P
SMILESN(C([C@@H](CCP(=O)(O)O)N)=O)C1=CC(CCCCCC)=CC=C1
TargetApoptosis
SolubilityIn Vitro: DMSO : 22.22 mg/mL (64.90 mM; Ultrasonic )

Bioactivity

In Vivo
W146 (5 mg/kg, ip, prior to AMD3100 administration) pre-treatment shows approximately 8-fold increase of KSL-HSPC mobilization, measured by the CFU-G/M colony forming assays, compared to that in mice treated with AMD3100 aloneThe W146-mediated augmentation of KSL-HSPC mobilization is specific, because pretreatment of mice with W140 is unable to produce any effect on AMD3100-stimulated KSL-HSPC mobilization. Injections of W146, W140, JTE013, or Cay10444 do not alter the basal WBC count in mice. Animal model: Mice (4-6-week-old). Dosage: 5 mg/kg. Administration: IP, 1 hour prior to AMD3100 (ADM) administration. Result: Significantly increased in KSL-HSPC mobilization compared to that in mice pretreated with dextran followed by AMD3100 administration.
In Vitro
W146 is a S1PR1 antagonist with a Ki of ~70-80 nM. W146 pretreatment significantly increases activated cleaved caspase-3 levels. The reduced EPCs apoptosis which induced by S1P is completely abolished after treatment with W146.

Storage & Handling

StorageStorage temperature: -20°C. Stability: ≥ 2 years
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Protocol Information

W146 (orb2901680)

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100 mg
500 mg
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¥ 4,420.00
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¥ 9,750.00
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¥ 16,380.00
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¥ 31,330.00
50 mg
¥ 48,100.00