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ZBP1 Antibody
SKU: orb1238568
Featured
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 9
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | ZBP1 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human ZBP1.The immunogen is located within the last 50 amino acids of ZBP1. |
| Target | ZBP1 |
| Molecular Weight | Predicted: 39, 45 kDa Observed: 42 kDa |
| Purification | ZBP1 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | ZBP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ZBP1 Antibody: DAI, DLM1, DLM-1, C20orf183, Z-DNA-binding protein 1, Tumor stroma and activated macrophage protein DLM-1
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WB Validation in Human Tonsil. Loading: 10 μg of lysate. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Brain. Loading: 10 μg of lysates per lane. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Brain. Loading: 10 μg of lysates per lane. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Rat Brain. Loading: 10 μg of lysate. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in A549 Cells. Loading: 10 μg of lysate. Antibodies: ZBP1 orb1238568, 1 μg/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of ZBP1 in Human Tonsil. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZBP1 antibody (orb1238568) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of ZBP-1 in Mouse Brain. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ZBP1 antibody (orb1238568) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of ZBP1 in Human K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 labeling ZBP1 with orb1238568 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of ZBP1 in Rat Brain. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling ZBP1 with orb1238568 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ZBP1 Antibody (orb1238568)
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