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ZNF239 Antibody

SKU: orb1826279

Description

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.

Images & Validation

Tested ApplicationsFACS
Dilution RangeFlow cytometry: 1-2ug/million cells
ReactivityHuman
Application Notes
Optimal dilution of the ZNF239 antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1
Clone No.PCRP-ZNF239-2A10
ImmunogenA recombinant partial protein (within amino acids 83-233) from the human protein was used as the immunogen for the ZNF239 antibody.
PurificationProtein A/G affinity
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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ZNF239 Antibody

Flow cytometry testing of PFA-fixed human HeLa cells with ZNF239 antibody (clone PCRP-ZNF239-2A10) followed by goat anti-mouse IgG-CF488 (blue), Red = unstained cells.

ZNF239 Antibody

SDS-PAGE analysis of purified, BSA-free ZNF239 antibody (clone PCRP-ZNF239-2A10) as confirmation of integrity and purity.

ZNF239 Antibody

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using ZNF239 antibody (clone PCRP-ZNF239-2A10). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

UniProt Details

No UniProt data available

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Protocol Information

FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
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ZNF239 Antibody (orb1826279)

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Available Sizes

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100 μg
¥ 9,750.00
DispatchUsually dispatched within 5-10 working days
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