Cart summary

You have no items in your shopping cart.

CD23 Antibody

SKU: orb248902

Description

The CD23 Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against Native purified human CD23. Purification: The antibody was purified by affinity chromatography.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsFC, IF
Dilution rangeIF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
ReactivityHuman
Application Notes
1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
Clone No.EBVCS-5
ImmunogenNative purified human CD23.
PurificationThe antibody was purified by affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceMouse IgG1 kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
DisclaimerFor research use only

Alternative Names

CD23A; CLEC4J; FCE2; IGEBF; Low affinity immunoglobulin epsilon Fc receptor; BLAST-2; C-type lectin domain family 4 member J; Fc-epsilon-RII; Immunoglobulin E-binding factor; Lymphocyte IgE receptor; CD antigen CD23)

Similar Products

  • CD23/FCER2 Antibody [orb182383]

    ELISA,  FC,  ICC,  IF,  IHC,  IHC-Fr,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  • Coronin 1a/TACO/CORO1A Antibody [orb745941]

    ELISA,  FC,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
  • FCER2 Antibody [orb1410535]

    IHC

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg (without BSA and Azide), 20 μg, 100 μg
  • FCER2 Antibody [orb1410377]

    IHC

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg, 100 μg (without BSA and Azide)
  • EIF2B1 Antibody [orb669081]

    FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

CD23 Antibody

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD23 Antibody, followed by anti-mouse IgG PE.

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Documents Download

Datasheet
Product Information
Download

Request a Document

Protocol Information

FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol

CD23 Antibody (orb248902)

  • Star
  • Star
  • Star
  • Star
  • Star
  • 0.0
Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

Login to Submit a Review

No reviews yet

Available Sizes

Select a size below

30 μl
¥ 1,430.00
50 μl
¥ 1,820.00
100 μl
¥ 2,340.00
200 μl
¥ 3,640.00
DispatchUsually dispatched within 5-10 working days
Bulk Enquiry