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Anti-CD40 [chi220]
SKU: orb758981
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 2
| Tested Applications | Blocking |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | chi220 |
| Immunogen | This antibody was generated by immunizing female BALB/c mice with a fusion protein containing extracellular domain of human CD40 fused to the hinge CH2 and CH3 domains of a murine IgG2b antibody. |
| Target | CD40 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−TNFSF5; CDw40; TRAP; p50; Bp50; CD40L receptor; Tumor necrosis factor ligand superfamily member 5; B-cell surface antigen CD40; T-cell antigen Gp39; TNF-related activation protein.
Similar Products
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![Anti-CD40 [chi220]](/images/pub/media/catalog/product/NewWebsite/35/orb758982_1.png)
![Anti-CD40 [chi220]](/images/pub/media/catalog/product/NewWebsite/35/orb758982_2.png)
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![Anti-CD40 [chi220]](/images/pub/media/catalog/product/NewWebsite/35/orb758981_1.png)
Flow cytometry using the anti-CD40 antibody chi220. Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of chi220 (orb758982, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
![Anti-CD40 [chi220]](/images/pub/media/catalog/product/NewWebsite/35/orb758981_2.png)
Immunofluorescence staining of fixed Daudi cells with anti-CD40 antibody chi220. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of chi220 (orb758982) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758982, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.
Quick Database Links
Gene Symbol
CD40
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
Anti-CD40 [chi220] (orb758981)
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