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HLA-DR/DP Antibody
SKU: orb43864
Description
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| Tested Applications | FC, IHC-P, IP, WB |
|---|---|
| Reactivity | Human, Porcine |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a |
| Clone No. | HL-38 |
| Immunogen | Raji Burkitt's lymphoma cell line |
| Target | HLA-DR/DP |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Flow cytometry analysis (surface staining) of human peripheral blood with anti-HLA-DR+DP (HL-38) PE.
Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-HLA-DR+DP (HL-38).
Anti-HLA-DR/DP (clone HL-38) works in WB application under reducing and non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with mouse IgG2a monoclonal antibody HL-38 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. HLA-DR/DP molecules were detected at ~25 kDa in Raji cell line.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IP
Immunoprecipitation
HLA-DR/DP Antibody (orb43864)
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