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HSP27 Antibody
SKU: orb1474330
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution range | WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) |
| Reactivity | Human, Monkey |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | Purified recombinant human Hsp27 protein fragments expressed in E.coli. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Disclaimer | For research use only |
Alternative Names
−HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27
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Western blot analysis of HSP27 expression in Hela (A), Hela (B), A431 (C), COS7 (D), Lncap (E), MCF7 (F) whole cell lysates. (Predicted band size: 23 kD; Observed band size: 27 kD)
Immunofluorescent analysis of HSP27 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
HSP27 Antibody (orb1474330)
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