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CD261 Antibody

SKU: orb154377

Description

Mouse monoclonal antibody to CD261 which is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to activated CD261 which is expressed in most human tissues including spleen, peripheral blood leucocytes, thymus and in a variety oFT tumour-derived cell lines. It plays a role in inducing cell death. The binding oFT TRAIL to DR4 triggers the activation oFT pro-caspases 8 and 10, leading to apoptosis.

Images & Validation

Tested ApplicationsFC, ICC, IP
ReactivityHuman
Application Notes
Flow cytometry: Recommended dilution: 1-4 μg/ml.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1
Clone No.DR-4-02
ImmunogenFusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.
TargetCD261
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

DR4, APO2, TNFRSF10A, TRAILR1, TRAIL-R1

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CD261 Antibody

Flow cytometry analysis of TRAIL-R1 expression on the surface of hematopoietic cell lines. Cells were stained with purified anti-TRAIL-R1 antibodies followed by Goat anti-mouse IgG PE.

CD261 Antibody

Immunocytochemistry staining (confocal microscopy) of HeLa human cervix carcinoma cell line transfected with TRAIL-R1 expression plasmid using anti-human TRAIL-R1 (DR-4-02).

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

FC
Flow Cytometry
View Protocol
ICC
Immunocytochemistry
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IP
Immunoprecipitation
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CD261 Antibody (orb154377)

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0.1 mg
¥ 4,550.00
DispatchUsually dispatched within 5-10 working days weeks.
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