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Anti-IL-18 Antibody Discontinued

SKU: orb378127

Description

Rabbit polyclonal antibody to IL18.

Research Area

Immunology

Images & Validation

Tested ApplicationsIF, IP, WB
Dilution RangeWB: 1:500-2000
ReactivityHuman, Mouse, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
ImmunogenRecombinant full length protein of human IL-18
TargetIL18
PurificationThe antibody was purified by immunogen affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

IGIF; IL1F4; Interleukin-18; IL-18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma

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Anti-IL-18 Antibody

Western blot analysis of IL-18 expression in Hela (A) whole cell lysates. (Predicted band size: 21; 22 kD; Observed band size: 22 kD)

Anti-IL-18 Antibody

Immunofluorescent analysis of IL-18 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol
IP
Immunoprecipitation
View Protocol

Filter by Applications

Filter by Species

Alireza Barati 1 2, Sadegh Moghimi 3, Kiana Taghavi Zanjani 1, Mojde Rohani 4, Mehri Sohrabi Hesar 1, Arian Arfaie 1, Mohadese Ghezelche Khamsiyan 1, Javad Mahmoudi 2, Saeed Sadigh-Eteghad Acute Administration of Edaravone Improves Cognitive Impairment in a Mouse Model of mPFC Ischemia: Crosstalk Between Necroptosis, Neuroinflammation, and Antioxidant Defense Mol Neurobiol ., (2024)

Applications

IF

Reactivity

Mouse

Anti-IL-18 Antibody (orb378127)

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