TCR alpha/beta Antibody

SKU: orb154386

Description

Mouse monoclonal antibody to TCR alpha/beta which recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex.

Images & Validation

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Tested Applications	FC, ICC, IHC-Fr, IHC-P, IP, WB
Reactivity	Primate, Rat
Application Notes	Flow cytometry: Recommended dilution: 1-5 μg/ml.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1 kappa
Clone No.	R73
Immunogen	Rat T blasts and erythrocytes
Target	TCR alpha/beta
Purification	Purified by protein-A affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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TCRA/B

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Separation of rat TCR alpha/beta positive thymocytes (red-filled) from TCR alpha/beta negative thymocytes (black-dashed) in flow cytometry analysis (surface staining) of rat thymocyte suspension stained using anti-rat TCR alpha/beta (R73) purified antibody (concentration in sample 1.6 μg/ml) GAM APC.

Flow cytometry surface staining pattern of rat thymocyte suspension stained using anti-rat TCR alpha/beta (R73) purified antibody (concentration in sample 1.6 μg/ml) GAM APC.

Quick Database Links

Gene Symbol

TCR alpha/beta

NCBI Gene ID

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Flow Cytometry

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ICC

Immunocytochemistry

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Immunoprecipitation

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