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ATF6 Antibody
SKU: orb1239223
Description
Research Area
Cell Biology, Signal Transduction
Images & Validation
−Item 1 of 7
| Tested Applications | ELISA, ICC, IF, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | ATF6 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ATF6.The immunogen is located within amino acids 470 - 520 of ATF6. |
| Target | ATF6 |
| Purification | ATF6 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | ATF6 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, cAMP-dependent transcription factor ATF-6 alpha
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Western blot analysis of ATF6 in EL4 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 µg/mL.
Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 µg/mL.
Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 µg/mL.
Immunohistochemistry of ATF6 in human breast tissue with ATF6 antibody at 5 µg/ml.
Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 µg/ml. Green: ATF6 Antibody (orb1239223) Blue: DAPI staining.
Immunohistochemistry of ATF6 in human pancreas tissue with ATF6 antibody at 5 µg/ml.
Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 µg/ml. Green: ATF6 Antibody (orb1239223) Blue: DAPI staining.
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ATF6 Antibody (orb1239223)
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